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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 464-832 mapping at the C-terminus of p300/CBP-associated factor (PCAF) of human origin
- recommended for detection of PCAF and GCN5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- TransCruz reagent for ChIP application, sc-8999 X, 200 µg/0.1 ml
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PCAF Background Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.
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Visualizza come altri ricercatori hanno utilizzato l'anticorpo PCAF (H-369): sc-8999 e/o i coniugati per l'anticorpo PCAF (H-369).
32 total citations Loading citations.
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PCAF (H-369)
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ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217, NCoA-3 (C-20): sc-7216, SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096, SRC-1 (M-20): sc-6098, GRIP1 (M-343): sc-8996, PCAF (C-16): sc-6300 and PCAF (H-369): sc-8999. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67. PCAF (H-369): sc-8999. Western blot analysis of His-tagged human recombinant PCAF.
ChIP analysis of recruitment of CBP, p300, PCA and BRG1 to the IL-2α (CD25) promoter in vivo. Antibodies tested include CBP (A-22): sc-369, p300 (N-15): sc-584, PCAF (H-369): sc-8999, and Brg-1 (H-88): sc-10768. CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag -/- thymocytes or Rag -/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press. PCAF (H-369): sc-8999. Western blot analysis of PCAF expression in K-562 (A) and BJAB (B) whole cell lysates.
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