 |
- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to an amino acid sequence containing phosphorylated Ser 10 of Histone H3 of human origin
- recommended for detection of Histone H3 of mouse, rat, human, Drosophila melanogaster and Xenopus laevis origin phosphorylated at Ser 10 by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-8656 P
- TransCruz reagent for ChIP application, sc-8656 X, 200 µg/0.1 ml
|
|
| |
Informazioni ordiniCitazioni prodotti
Recommended Support Products:
(click button of application of choice)
Histone H3 Background Information
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure (1,2). The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome (2). The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA (3). Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase (4,5). The two major sites of phosphorylation are the mitosis-specific site Ser10, and Ser28, both of which are extensively phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone H3 (6). The nucleosome structure of histone H3 promotes N-terminal phosphorylation in vitro (6).
| p-Histone H3 (Ser 10)-R Citazioni prodotti |
Visualizza come altri ricercatori hanno utilizzato l'anticorpo p-Histone H3 (Ser 10)-R: sc-8656-R e/o i coniugati per l'anticorpo p-Histone H3 (Ser 10)-R.
24 total citations Loading citations.
|
| |
|
|
|
p-Histone H3 (Ser 10)-R
Clicca sull'immagine per ingrandirla
|
Western blot analysis of Histone H3 phosphorylation in HeLa (A,C) and HeLa + Calyculin A (B,D) whole cell lysates. Blots were probed with p-Histone H3 (Ser 10)-R: sc-8656-R preincubated with cognate phosphorylated (A,B) and unphosphorylated (C,D) peptide. Western blot analysis of Histone H3 phosphorylation in HeLa (A,C) and HeLa + calcyculin (B,D) whole cell lysates. Blots were probed with p-Histone H3 (Ser 10)-R: sc-8656-R preincubated with cognate phosphorylated (A,B) and unphosphorylated (C,D) peptide. p-Histone H3 (Ser 10)-R: sc-8656-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded E14.5 embryonic mouse lung tissue showing nuclear localization. Kindly provided by Whitsett Laboratory, Division of Pulmonary Biology and Neonatology at Cincinnati Childrens Hospital Medical Center. p-Histone H3 (Ser 10)-R: sc-8656-R. Western blot analysis of Histone H3 phosphorylation in untreated (A), calyculin A treated (B) and calyculin A and lambda protein phosphatase treated (C) HeLa whole cell lystates. Western blot analysis of Histone H3 phosphorlation in untreated (A,H), Trichostatin A treated (B,I) calyculin A treated (C,J), Trichostatin A and calyculin A treated (D,K), Trichostatin A and lambda protein phosphatase treated (E,L), calyculin A and lambda protein phosphatase treated (F,M) and Trichostatin A, calyculin A and lambda protein phosphatase treated (G,N) NIH/3T3 whole cell lysates. Antibodies tested include p-Histone H3 (Ser 10)-R: sc-8656-R (A,B,C,D,E,F,G) and Histone H3 (FL-136): sc-10809 (H,I,J,K,L,M,N). |
|
Download
|
|
