 |
- mouse monoclonal IgG1; 200 µg/ml
- specific for an epitope mapping between amino acids 2420-2442 at the C-terminus of CBP of human origin (identical to corresponding mouse sequence)
- recommended for detection of CBP p265 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine, bovine, porcine and avian
- blocking peptide, sc-7300 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-7300 X, 200 µg/0.1 ml
|
|
| |
Informazioni ordiniCitazioni prodotti
Recommended Support Products:
(click button of application of choice)
| |
| Specie |
Nome del gene |
Codice del gene |
Locus cromosoma |
Isoform (mRNA) Accession # |
codice accesso proteina |
Numero d'ordine |
| Umano |
CREBBP |
1387 |
16p13.3 |
NM_004380 |
Q92793
|
600140 |
| Mouse |
Crebbp |
12914 |
16 A1 |
|
P45481
|
N/A |
| |
CBP Background Information
Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression, even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.
| CBP (C-1) Citazioni prodotti |
Visualizza come altri ricercatori hanno utilizzato l'anticorpo CBP (C-1): sc-7300 e/o i coniugati per l'anticorpo CBP (C-1).
42 total citations Loading citations.
|
| |
|
|
|
CBP (C-1)
Clicca sull'immagine per ingrandirla
|
|
CBP (C-1): sc-7300. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
CBP (C-1): sc-7300. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
Western blot analysis of CBP expression in HeLa (A) and Jurkat (B) nuclear extracts. Antibodies tested include CBP (C-1): sc-7300 (A) and CBP (C-20): sc-583 (B). ChIP analysis of coactivator recruitment on Cyclin D2 promoter in C2C12 cells treated with LiCl and serum. Antibodies tested include β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496, β-catenin (E-5): sc-7963, Tip60 (N-17): sc-5725, TRRAP (T-17): sc-5405, TRRAP (Y-18): sc-12375, TRRAP (F-20): sc-12376, TRRAP (H-300): sc-11411, CBP (A-22): sc-369, CBP (C-20): sc-583, CBP (451): sc-1211, CPB (C-1): sc-7300, p300 (H-272): sc-8981, p300 (N-15): sc-584 and p300 (C-20): sc-585. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
ChIP analysis of p52 promoter occupancy in murine p19 cells in response to activation by estradiol (E2). Antibodies tested include ERα (MC-20): sc-542, ERα (H-184): sc-7207, CBP (A-22): sc-369, CBP (C-1): sc-7300, p/CIP (F2): sc-5305, p/CIP (M-397): sc-9119, RIP140 (H-300): sc-8997 and HDAC3 (N-19): sc-11417. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526. ChIP analysis of cofactor occupancy dynamics on the ICAM1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038, PCAF (C-16): sc-6300, PCAF (H-369): sc-8999, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211, p300 (C-20): sc- sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67. CBP siRNA (h): sc-29244. Immunofluorescence staining of methanol-fixed, control HeLa (A) and CBP siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with CBP (C-1): sc-7300. CBP (C-1): sc-7300. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing nuclear staining of ovarian stromal cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
CBP (C-1): sc-7300. Western blot analysis of CBP expression in Jurkat (A) and A-431 (B) nuclear extracts.
|
|
Download
|
|
