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- goat polyclonal IgG, 200 µg/ml
- epitope mapping at the N-terminus of TIP60 of human origin
- recommended for detection of TIP60 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine and porcine
- blocking peptide, sc-5725 P
- TransCruz reagent for ChIP application, sc-5725 X, 200 µg/0.1 ml
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TIP60 Background Information
MOZ (monocytic leukemia zinc finger protein) is a chromatin-associated histone acetyltransferase (HAT) that regulates chromatin remodeling and transcription. The MOZ gene was initially isolated as a consequence of two variant translocations that were identified in a distinct subtype of acute myeloid leukemias and resulted in the formation of MOZ fusion proteins. These fusions involve the HAT domain of MOZ with the activation domain of either transcriptional coactivator protein TIF2/GRIP1 or CBP, and lead to enhanced transcriptional activation by a mechanism involving aberrant histone acetylation. Additional MOZ related proteins, including MORF (MOZ related factor) and TIP60 (TAT interacting proteins 60), share significant similarities with MOZ including the putuative HAT domain. MORF also contains a strong transcriptional repression domain at its N terminus and a highly potent activation domain at the C terminus, suggesting that MORF has both HAT activity and contributes to the regulation of transcriptional activation. TIP60 was originally identified as a coactivator for the HIV TAT protein and also functions as a nuclear hormone receptor coactivator that enhances ligand dependent steroid receptor-mediated transactivation involving the androgen, estrogen and progesterone receptors.
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Visualizza come altri ricercatori hanno utilizzato l'anticorpo TIP60 (N-17): sc-5725 e/o i coniugati per l'anticorpo TIP60 (N-17).
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TIP60 (N-17)
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TIP60 (N-17): sc-5725. Western blot analysis of TIP60 expression in SK-BR-3 (A), KNRK (B) and IMR-32 (C) nuclear extracts.
ChIP analysis of coactivator recruitment on Cyclin D2 promoter in C2C12 cells treated with LiCl and serum. Antibodies tested include β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496, β-catenin (E-5): sc-7963, Tip60 (N-17): sc-5725, TRRAP (T-17): sc-5405, TRRAP (Y-18): sc-12375, TRRAP (F-20): sc-12376, TRRAP (H-300): sc-11411, CBP (A-22): sc-369, CBP (C-20): sc-583, CBP (451): sc-1211, CPB (C-1): sc-7300, p300 (H-272): sc-8981, p300 (N-15): sc-584 and p300 (C-20): sc-585. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included RORα (C-16): sc-6062 (B), TIP60 (N-17): sc-5725 (C), CBP (A-22): sc-369 and CBP (C-20): sc-583 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131. ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included RORα (C-16): sc-6062 (B), TIP60 (N-17): sc-5725 (C), CBP (A-22): sc-369 and CBP (C-20): sc-583 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131. ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included RORα (C-16): sc-6062 (B), TIP60 (N-17): sc-5725 (C), CBP (A-22): sc-369 and CBP (C-20): sc-583 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131. TIP60 (N-17): sc-5725. Western blot analysis of TIP60 expression in non-transfected: sc-117752 (A) and mouse TIP60 transfected: sc-124076 (B) 293T whole cell lysates. |
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