epitope mapping within an internal region of HiNF-P of human origin
recommended for detection of HiNF-P of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
TransCruz reagent for Gel Supershift and ChIP applications, sc-49818 X, 200 µg/0.1 ml
HiNF-P Background Information HiNF-P is a critial transcription factor which is necessay for E2F-independent activation of the Histone H4 multigene family. HiNF-P associates with conserved H4 cell cycle regulatory sequences in vivo. Antisense inhibition of HiNF-P reduces endogenous Histone H4 gene expression. HiNF-P utilizes NPAT/p220, a substrate of the cyclin E/cyclin-dependent kinase 2 (CDK2) kinase complex, as a crucial coactivator to amplify Histone H4 gene transcription. The biological role of HiNF-P is reflected by impeded cell cycle progression into S phase upon antisense-mediated reduction of HiNF-P levels. Research indicates that HiNF-P is the key link in a linear signaling pathway that is initiated with the growth factor-dependent induction of cyclin E/
CDK2 kinase activity at the restriction point and culminates in the activation of Histone H4 genes through HiNF-P at the G1/S phase transition.
HiNF-P (A-19)
Clicca sull'immagine per ingrandirla
HiNF-P (A-19): sc-49818. Western blot analysis of HiNF-P expression in mouse brain tissue extract.
