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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-372-G
- epitope mapping at the C-terminus of NFκB p65 of human origin
- recommended for detection of NFκB p65 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-372 P
- agarose conjugate for IP studies, sc-372 AC, 500 µg/0.25 ml agarose
- TransCruz reagent for Gel Supershift and ChIP applications, sc-372 X, 200 µg/0.1 ml
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NFκB p65 Background Information
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NF˚B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp ˚B sequence in the immunoglobulin k light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkB is activated and NFkB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
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Visualizza come altri ricercatori hanno utilizzato l'anticorpo NFκB p65 (C-20): sc-372 e/o i coniugati per l'anticorpo NFκB p65 (C-20).
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NFκB p65 (C-20)
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Western blot analysis of NFκB p65 in NIH/3T3 (A,D), A-431 (B,E) and K-562 (C,F) whole cell lysates. Antibodies tested include NFκB p65 (C-20): sc-372 (A-C) and NFκB p65 (C-20)-G: sc-372-G (D-F). NFκB p65 (C-20)-G: sc-372-G. Western blot analysis of NFκB p65 expression in K-562 (A), A-431 (B) and NIH/3T3 (C) whole cell lysates. NFκB p65 (C-20): sc-372. (A) Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining. (B) NFκB p65 (C-20)-G: sc-372-G. Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining. ChIP analysis of cofactor occupancy dynamics on the ICAM1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, Bcl-3 (C-14): sc-185, Bcl-3 (H-146): sc-13038, PCAF (C-16): sc-6300, PCAF (H-369): sc-8999, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211, p300 (C-20): sc- sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67. NFκB p65 (C-20): sc-372. Western blot analysis of NFκB p65 expression in non-transfected: sc-117752 (A) and mouse NFκB p65 transfected: sc-122027 (B) 293T whole cell lysates. NFκB p65 (C-20)-G: sc-372-G. Western blot analysis of NFκB p65 expression in non-transfected: sc-117752 (A) and mouse NFκB p65 transfected: sc-122027 (B) 293T whole cell lysates. NFκB p65 (C-20)-G: sc-372-G. Western blot analysis of NFκB p65 expression in NIH/3T3 whole cell lysate. NFκB p65 (C-20): sc-372. Western blot analysis of NFκB p65 expression in non-transfected 293T: sc-117752 (A), human NFκB p65 transfected 293T: sc-112033 (B) and K-562 (C) whole cell lysates. NFκB p65 (C-20)-G: sc-372-G. Western blot analysis of NFκB p65 expression in non-transfected 293T: sc-117752 (A), human NFκB p65 transfected 293T: sc-112033 (B) and K-562 (C) whole cell lysates. Western blot analysis of NFκB p65 phosphorylation in untreated (A,D), TNFα and calyculin A treated (B,E) and TNFα, calyculin and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-NFκB p65 (Ser 311): sc-33039 (A,B,C) and NFκB p65 (C-20): sc-372 (D,E,F). Western blot analysis of NFκB p65 phosphorylation in untreated (A,C), and lambda protein phosphatase treated (B,D) K-562 whole cell lysates. Antibodies tested include p-NFκB p65 (Ser 311): sc-33039 (A,C) and NFκB p65 (C-20): sc-372 (B,D). NFκB p65 (C-20)-G: sc-372-G. Western blot analysis of NFκB p65 expression in HeLa (A), MIA PaCa-2 (B), T24 (C) and SK-BR-3 (D) whole cell lysates. NFκB p65 (C-20)-G: sc-372-G. Western blot analysis of NFκB p65 expression in non-transfected 293T: sc-117752 (A), mouse NFκB p65 transfected 293T: sc-122027 (B), Jurkat (C) and K-562 (D) whole cell lysates. |
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