- 25 KU
- ≥250 units/μL
- Genetically engineered endonuclease from Serratia marcescens. The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable.Unit definitionOne unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.
- >90% by SDS-PAGE
- supplied in 50% glycerol containing 50 mM Tris-HCl pH 8.0, 20 mM NaCl and 2 mM MgCl2
- The enzyme preparation is stable for 2 years when stored at –20°C.
DO NOT store at –70°C because freezing Benzonase Nuclease results in loss of activity.
- 1. Miller, M.D., et al. 1994. Nat. Struct. Biol. 1: 461-468. PMID: 7664065
2. Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-3287. PMID: 8078761
3. Ball, T.K., et al. 1992. Nucleic Acids Res. 20: 4971-4974. PMID: 1329033
4. Ball, T.K., et al. 1987. Gene. 57: 183-192. PMID: 3319779
5. Janning, P., et al. 1994. Rapid Commun. Mass Spectrom. 8: 1035-1040. PMID: 7696699
6. Molloy, M.P., et al. 1998. Electrophoresis. 19: 837-844. PMID: 9629924
7. Herbert, B.R., et al. 1998. Electrophoresis. 19: 845-851. PMID: 9629925
- For Research Use Only
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