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- available in 100,000 units in 0.25ml (sc-200312) and 20,000 units in 0.05ml (sc-200312A) sizes
- Bacteriophage λ, recombinant (E. coli)
- Lambda Phosphatase can be used to release phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins; also active on phosphorylated histidine residues
- 1 mL of 10X Reaction buffer and 1 mL of 10X MnCl2 included with each size
- Lambda Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. The enzyme is inhibited by vanadate ions and can be heat inactivated at 65º C for 1 hour in the presence of 50 mM EDTA.
- Purity: minimum 95% (SDS-PAGE) with no detectable protease, DNase, or RNase activity
- supplied as a solution containing approximately 400,000 units of protein per ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.01% BRIJ 35 and 50% glycerol.
- Unit Definition: One unit will hydrolyze 1 nmole of p-nitophenyl phosphate per minute at 30º C and pH 7.5
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Lambda Phosphatase
Clicca sull'immagine per ingrandirla
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Western blot analysis of FAK phosphorylation in non-transfected: sc-117752 (A,D), untreated human FAK transfected: sc-114600 (B,E) and lambda protein phosphatase (sc-200312A) treated human FAK transfected: sc-114600 (C,F) 293T whole cell lysates. Antibodies tested include p-FAK (Tyr 577)-R: sc-16665-R (A,B,C) and FAK (C-903): sc-932 (D,E,F). |
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