UBE2D1 Background Information UBE2D1 (ubiquitin-conjugating enzyme E2D1 or UBC5A), UBE2D2 (ubiquitin-conjugating enzyme E2D2 or UBC5B), and UBE2D3 (ubiquitin-conjugating enzyme E2D3 or UBC5C) are E2 ubiquitin-conjugating enzymes, components of the protein ubiquitination pathway. Protein ubiquitination covalent modification targets proteins for 26 S proteasome-dependent degradation. 3 classes of enzymes influence the conjugation mechanism of ubiquitin to protein. E1 ubiquitin-activating enzymes mediate ATP-dependent charging of ubiquitin via formation of a high energy thiol ester bond between the C-terminus of ubiquitin and a cysteine within itself. Thiol ester-linked ubiquitin is then transferred from E1 to a cysteine residue in an E2 ubiquitin-conjugating enzyme. E2 enzymes in conjunction with E3 ubiquitin-protein ligases transfer ubiquitin monomers and polyubiquitin chains to the substrate target protein, where stable isopeptide linkages are formed.
UBE2D1 (C-22)
Clicca sull'immagine per ingrandirla
UBE2D1 (C-22): sc-130283. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.
UBE2D1 (C-22): sc-130283. Western blot analysis of UBE2D1 expression in Jurkat whole cell lysate.
UBE2D1 (C-22): sc-130283. Western blot analysis of UBE2D1 expression in non-transfected 293T: sc-117752 (A), mouse UBE2D1 transfected 293T: sc-124410 (B) and NIH/3T3 (C) whole cell lysates.
UBE2D1 (C-22): sc-130283. Western blot analysis of UBE2D3 expression in non-transfected: sc-110760 (A) and human UBE2D3 transfected: sc-110785 (B) 293 whole cell lysates.
