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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 1-136 representing full length Histone H3 of human origin
- recommended for detection of Histone H3 of broad mammalian, Drosophila, Xenopus and C. elegans origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- TransCruz reagent for ChIP application, sc-10809 X, 200 µg/0.1 ml
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Histone H3 Background Information
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
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Visualizza come altri ricercatori hanno utilizzato l'anticorpo Histone H3 (FL-136): sc-10809 e/o i coniugati per l'anticorpo Histone H3 (FL-136).
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Histone H3 (FL-136)
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Histone H3 (FL-136): sc-10809. Western blot analysis of human recombinant Histone H3 fusion protein.
Histone H3 (FL-136): sc-10809. Western blot analysis of Histone H3 expression in sodium butyrate-treated HeLa whole cell lysate.
Histone H3 (FL-136): sc-10809. Western blot analysis of Histone H3 expression in 293 (A) and HeLa (B) whole cell lysates.
Histone H3 (FL-136): sc-10809. Western blot analysis of Histone H3 expression in NIH/3T3 whole cell lysate.
Histone H3 (FL-136): sc-10809. Western blot analysis of Histone H3.3B expression in non-transfected 293T: sc-117752 (A), human Histone H3.3B transfected 293T: sc-112017 (B) and HeLa (C) whole cell lysates. Histone H3 (FL-136): sc-10809. Western blot analysis of Histone H3.3B expression in non-transfected 293T: sc-117752 (A), mouse Histone H3.3B transfected 293T: sc-125446 (B) and HeLa (C) whole cell lysates. Histone H3 (FL-136): sc-10809. Western blot analysis of Histone H3.3A expression in non-transfected 293T: sc-117752 (A), human Histone H3.3A transfected 293T: sc-117359 (B) and HeLa (C) whole cell lysates. Histone H3 (FL-136): sc-10809. Western blot analysis of Histone H3.3A expression in non-transfected 293T: sc-117752 (A), human Histone H3.3A transfected 293T: sc-115484 (B) and HeLa (C) whole cell lysates. Histone H3 (FL-136): sc-10809. Western blot analysis of Histone H3.3A expression in non-transfected 293T: sc-117752 (A), human Histone H3.3A transfected 293T: sc-159580 (B) and NTERA-2 cl.D1 (C) whole cell lysates. Western blot analysis of Histone H3 acetylation in untreated (A,H), Trichostatin A treated (B,I) calyculin A treated (C,J), Trichostatin A and calyculin A treated (D,K), Trichostatin A and lambda protein phosphatase treated (E,L), calyculin A and lambda protein phosphatase treated (F,M) and Trichostatin A, calyculin A and lambda protein phosphatase treated (G,N) NIH/3T3 whole cell lysates. Antibodies tested include Ac-Histone H3 (AH3-120): sc-56616 (A,B,C,D,E,F,G) and Histone H3 (FL-136): sc-10809 (H,I,J,K,L,M,N).
Western blot analysis of Histone H3 phosphorlation and acetylation in untreated (A,H), Trichostatin A treated (B,I) calyculin A treated (C,J), Trichostatin A and calyculin A treated (D,K), Trichostatin A and lambda protein phosphatase treated (E,L), calyculin A and lambda protein phosphatase treated (F,M) and Trichostatin A, calyculin A and lambda protein phosphatase treated (G,N) NIH/3T3 whole cell lysates. Antibodies tested include p-Ac-Histone H3 (Ser 11/Lys 15): sc-33361 (A,B,C,D,E,F,G) and Histone H3 (FL-136): sc-10809 (H,I,J,K,L,M,N).
Western blot analysis of Histone H3 phosphorlation in untreated (A,H), Trichostatin A treated (B,I) calyculin A treated (C,J), Trichostatin A and calyculin A treated (D,K), Trichostatin A and lambda protein phosphatase treated (E,L), calyculin A and lambda protein phosphatase treated (F,M) and Trichostatin A, calyculin A and lambda protein phosphatase treated (G,N) NIH/3T3 whole cell lysates. Antibodies tested include p-Histone H3 (Ser 10)-R: sc-8656-R (A,B,C,D,E,F,G) and Histone H3 (FL-136): sc-10809 (H,I,J,K,L,M,N). Western blot analysis of Histone H3 phosphorylation in untreated (A,D), calyculin A treated (B,E) and calyculin A and lambda protein phosphatase ( sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested is p-Histone H3 (C-2): sc-374669 (A,B,C) and Histone H3 (FL-136): sc-10809 (D,E,F). |
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