shRNA Plasmid Transfection Medium, shRNA and Lentiviral Particle Gene Silencers
shRNA Plasmid Transfection Medium: sc-108062
20 ml
reduced-serum medium suitable for addition to shRNA suspension and siRNA Transfection Reagent, immediately prior to cell transfection
modification of Eagle’s Minimal Essential Medium, buffered with HEPES and sodium bicarbonate, and supplemented with hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements and growth factors; protein level is minimal with insulin and transferrin being the only protein supplements; phenol red included at a reduced concentration as a pH indicator