0.2 ml; sufficient for 50-100 transfections (using 6-well plates)
enables highly efficient shRNA Plasmid DNA transfection in a variety of cell lines including CHO-K1, COS, LNCaP, NIH/3T3, 293, T24, C2C12, SF-9, primary human keratiocytes, primary aortic smooth muscle, primary rabbit myoblasts, human bone marrow endothelial cells (HBMEC)
effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity