ASAHL Background Information ASAHL (N-acylsphingosine amidohydrolase (acid ceramidase)-like), also known as PLT or NAAA (N-acylethanolamine-hydrolyzing acid amidase), is a member of the choloylglycine hydrolase family and is widely expressed with predominant levels found in kidney and liver. ASAHL is structurally and functionally similar to Acid Ceramidase but exhibits low ceramide-hydrolyzing activity. Localizing to lysosomes, ASAHL functions in the hydrolyzation of bioactive N-acylethanolamines (NAEs) to ethanolamine and free fatty acids. Unlike FAAH (another NAE-hydrolyzing enzyme), ASAHL operates at an optimal pH of 4.5-5 and, once cleaved to its active form, exhibits a preference for N-palmitoylethanolamine and anandamide (N-arachidonoylethanolamine). ASAHL contains four glycosylation sites that are essential for stabilization of the enzyme and its activity is activated by dithiothreitol (DTT) and Triton X-100.
ASAHL (A19)
Clicca sull'immagine per ingrandirla
ASAHL (A19): sc-100470. Western blot analysis of ASAHL expression in NIH/3T3 whole cell lysate.
